Thermo Fisher Scientific MANEA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000
• Publications: -

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000
• Publications: -

Product Specifications

Product Specific Information

• Recommended positive controls: A431, HeLa, HepG2
• Predicted reactivity: Mouse (81%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005).

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.