Thermo Fisher Scientific TGIF1 Monoclonal Antibody (OTI1A6), TrueMAB
인간 TGIF1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB 및 IHC(P) 실험에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능합니다. 연구용으로 SMAD2 관련 전사 억제 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A6 |
| Immunogen | Full-length human recombinant protein of TGIF1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
TGIF is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators.
This homeodomain binds to the retinoid X receptor responsive element from the cellular retinol-binding protein II promoter.
TGIF functions as a transcriptional co-repressor of SMAD2 and may play a role in nuclear signaling during development and in adults.
Mutations in TGIF1 are associated with holoprosencephaly type 4, a structural anomaly of the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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