Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Brilliant Ultra Violet 496, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 585
• NovaFluor Blue 610-30S
• NovaFluor Blue 660-40S
• NovaFluor Blue 690
• NovaFluor Blue 760
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, and binds glycoproteins such as CD34, glycam-1, and MAdCAM-1. These interactions mediate lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) in peripheral lymphoid tissues.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry using human peripheral blood cells (5 µL/test, 0.25 µg/test)
• Recommended Cell Count: 10⁵–10⁸ cells/test
• Fluorochrome: Brilliant Ultra Violet™ 496 (BUV496), emission at 496 nm, for UV (355 nm) laser
• Light Sensitivity: Protect from light to prevent photo-oxidation
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when combining multiple Brilliant or polymer dye-conjugated antibodies

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor belonging to the selectin family. It consists of a C-type lectin domain, EGF-like domain, two short consensus repeat sequences, a spacer, transmembrane region, and cytoplasmic tail. It is constitutively expressed on most leukocytes, including lymphocytes (except some memory T cells), monocytes, and neutrophils.

For Research Use Only. Not for use in diagnostic procedures.