Thermo Fisher Scientific p14ARF Monoclonal Antibody (14P02 (DCS-240))

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

View 9 publications 9 publications

Immunoprecipitation (IP)

2 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Fruit fly, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

14P02 (DCS-240)

Immunogen

Recombinant p14ARF protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11001591

Product Specific Information

MA5-14260 targets p14ARF/p16beta in ICC/IF, IP, and WB applications and shows reactivity with Human samples.

The MA5-14260 immunogen is recombinant p14ARF protein.

Target Information

The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.