Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue / Yellow series
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays a key role in T-cell development and activation. Binding of RM4-5 is blocked by GK1.5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; ≤0.25 µg/test recommended
• Excitation / Emission: 407 nm / 649 nm (Violet laser)
• Light Sensitivity: Protect from light
• Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution; not compatible with methanol-based fixation
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when using multiple polymer dyes

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD4 is a co-receptor for MHC class II molecules and is primarily expressed on T helper cells, as well as monocytes, macrophages, and dendritic cells. It is involved in T-cell activation via association with the T-cell receptor complex and Lck kinase. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and other tissues. It also plays a role in neuronal damage during immune-mediated diseases.

For Research Use Only. Not for use in diagnostic procedures.