Thermo Fisher Scientific PUF60 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PUF60,uniProtId:Q9UHX1-1,ncbiNodeId:9606,antigenRange:243-542,antigenLength:559,antigenImageFileName:PA5-121029_PUF60_Q9UHX1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121029_PUF60_Q9UHX1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914601

Product Specific Information

Positive test controls include: THP-1, A-549, Mouse brain, Mouse thymus, Mouse spleen, Rat lung, Rat liver, Rat heart. The target is usually found in the following locations: Nucleus.

Immunogen sequence: DPTTGKHKGY GFIEYEKAQS SQDAVSSMNL FDLGGQYLRV GKAVTPPMPL LTPATPGGLP PAAAVAAAAA TAKITAQEAV AGAAVLGTLG TPGLVSPALT LAQPLGTLPQ AVMAAQAPGV ITGVTPARPP IPVTIPSVGV VNPILASPPT LGLLEPKKEK EEEELFPESE RPEMLSEQEH MSISGSSARH MVMQKLLRKQ ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA

Target Information

PUF60 encodes a protein that is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.