Invitrogen™

ProQuest™ Two-Hybrid System with Gateway™ Technology

The ProQuest™ Two-Hybrid System with Gateway™ Technology is an in vivo yeast-based system for identifying interactions between two proteins. This자세히 알아보기

The ProQuest™ Two-Hybrid System with Gateway™ Technology is an in vivo yeast-based system for identifying interactions between two proteins. This interaction reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media.
The ProQuest™ System features:
• Gateway™ Technology to allow rapid and easy generation of bait and prey constructs, and to facilitate downstream application
• Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
• Three different reporter genes (_HIS_3, _URA_3, and _lac_Z) with independent promoter regions to rapidly weed out false positives. _URA_3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid.

• A set of strong-to-weak Gateway™-based control interactions to evaluate results and assess the strength of the interaction being tested

사양

제품 유형Two-Hybrid System

리포터 유전자Beta-Gal (lacZ), HIS3, URA3

어세이Two-Hybrid Assay

발현 시스템Yeast

수량1 kit

제품라인Gateway™, ProQuest™

Unit SizeEach