Thermo Fisher Scientific Cul5 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot and freeze at -20°C or below for extended storage.
• Avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody reacts with human Cullin 5. Cross-reactivity is expected against mouse Cul5 based on a high degree of sequence homology. Cross-reactivity with other human cullins may occur.

Target Information

CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein, it may contribute to catalysis by positioning the substrate and ubiquitin-conjugating enzyme. The functional specificity of the E3 complex depends on the variable substrate recognition component. ECS(SOCS1) directs ubiquitination of JAK2 and may be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. It may also form a cell surface vasopressin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.