Thermo Fisher Scientific EGFR Monoclonal Antibody (OTI1A3), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR is associated with multiple cancer types and serves as a therapeutic target for biological and small molecule inhibitors.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ERbB family of receptor tyrosine kinases, including ERbB2, ERbB3, and ERbB4.
Ligand binding (e.g., EGF, TGF-α) induces receptor dimerization and autophosphorylation of cytoplasmic tyrosine residues, activating downstream signaling.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.