Thermo Fisher Scientific CellSensor NFκB-bla RA 1 Cell Line
NFκB 반응 요소에 기반한 베타-락타마제 리포터 유전자를 안정적으로 발현하는 RA-1 세포주. TNF 자극에 반응하며 NFκB 신호전달 연구 및 약물 스크리닝에 적합. DMSO 내성, 세포 수, 자극 시간 등 검증 완료.
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Thermo Fisher Scientific CellSensor™ NFκB-bla RA-1 Cell Line
The CellSensor™ NFκB-bla RA-1 cell line contains a beta-lactamase reporter gene under the control of the NFκB response element, stably integrated into Ramos 1 (RA-1) cells.
To construct this cell line, the NFκB-bla construct was transduced into RA-1 cells via lentivirus. Flow cytometry was used to isolate cells responsive to Tumor Necrosis Factor (TNF).
This cell line was validated for:
- DMSO tolerance
- Cell number
- Stimulation time
- Substrate loading time
- Z' and EC50 concentrations for TNF
The CellSensor™ NFκB-bla RA-1 cell line is responsive to TNF and can be used to probe NFκB signaling pathways, which are involved in the regulation of apoptosis, viral replication, tumorigenesis, inflammation, and various autoimmune diseases.
It can be adapted for high-throughput screening of agonists or antagonists using compound libraries. Candidate drugs can be tested for dose-response against this cell line.
Academic and non-profit customers, please inquire for special pricing.
제품 사양
| 항목 | 내용 |
|---|---|
| 어세이 입력 | Cell-based beta lactamase reporter gene |
| 셀 상태 | Dividing Cells |
| 제품 라인 | CellSensor™ |
| 수량 | 1 Vial |
| 타겟 입력 | TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway |
| System Type | CellSensor™ |
| Druggable Target | Signaling Pathway |
| Unit Size | Each |
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