Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Recombinant Rabbit Monoclonal Antibody (PS01-04)
Phospho-S6 (Ser235, Ser236) 항체로 Western blot, IHC, ICC/IF에 적합. 인간, 마우스, 랫트 반응성. 재조합 토끼 단클론 항체로 높은 특이성과 재현성 제공. 단백질 합성 및 세포 성장 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PS01-04 |
| Immunogen | Synthetic peptide within Human RPS6 aa 200–249 (phospho S235 + S236) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094158 |
Product Specific Information
- Positive control: NIH/3T3 cell lysate, THP-1 cell lysate, HeLa cell lysate, rat spleen tissue, HeLa
- Predicted band size: 29 kDa
- Subcellular Location: Cytosol, Endoplasmic reticulum, Nucleus
Target Information
Ribosomes are organelles responsible for protein synthesis, composed of a small 40S subunit and a large 60S subunit. These contain 4 RNA species and about 80 distinct proteins. The gene encoding this cytoplasmic ribosomal protein (RPS6) is part of the S6E family and serves as a major substrate for ribosomal protein kinases. Phosphorylation at five C-terminal serine residues is regulated by various stimuli such as growth factors, tumor-promoting agents, and mitogens, while dephosphorylation occurs during growth arrest. This protein may play a role in regulating cell growth and proliferation by selectively translating specific mRNA classes. Multiple processed pseudogenes of this gene are dispersed throughout the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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