Thermo Fisher Scientific COMT Polyclonal Antibody
COMT 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, IP 등 다양한 응용에 적합. 인간 시료 반응성, 항원 친화 크로마토그래피로 정제. 액상 형태, 4°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 221 and 271 of human COMT (Catechol-O-Methyltransferase) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
Target Information
Catechol-O-methyltransferase (COMT) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including dopamine, epinephrine, and norepinephrine. This O-methylation is a major degradative pathway for catecholamine neurotransmitters.
COMT also plays a key role in the metabolism of catechol drugs used to treat hypertension, asthma, and Parkinson’s disease.
It exists in two forms: soluble (S-COMT) and membrane-bound (MB-COMT), differing in their N-terminal regions. Several transcript variants arise from alternative translation initiation sites and promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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