Thermo Fisher Scientific IDH1 Monoclonal Antibody (GT1521)
IDH1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, ICC/IF, IP에 적합합니다. 인간, 마우스, 랫트에 반응하며, 단백질 G로 정제된 액상 항체입니다. 연구용으로 세포 내 IDH1 발현 확인에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1521 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IDH1 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735231 |
Product Specific Information
- Positive Control: 293T, A431, HeLa, HepG2, Huh-7, PC-12, Rat2, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, C2C12
- Predicted Reactivity: Zebrafish (85%), Xenopus laevis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two subclasses: one utilizing NAD(+) and the other NADP(+).
Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent (mitochondrial matrix) and two NADP(+)-dependent (one mitochondrial, one cytosolic).
The IDH1 gene encodes the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes, containing the PTS-1 peroxisomal targeting signal sequence.
This enzyme contributes to NADPH regeneration for intraperoxisomal reductions and cytoplasmic NADPH production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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