Thermo Fisher Scientific CPLX1/CPLX2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to amino acid residues 14-27 of rat Complexin-1 (Intracellular, N-terminus)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2857262

Product Specific Information

Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Peptide sequence is: (C)KDMGKML GGDEEKD

Homology: Complexin 1: Mouse, human amino acid residues are identical.Complexin 2: Rat, mouse, human - 13/14 amino acid residues identical.

Target Information

Complexins (1 through 4) are small cytosolic proteins that bind tightly to a complex of SNARE proteins to regulate synaptic vesicle membrane fusion via its interaction withsynaptotagmin. Complexins activate the quick release of neurotransmitters, triggered by calcium release. They also regulate the spontaneous release of neurotransmitters. Complexins are evolutionarily conserved in mammals, Drosophila, and Caenorhabditis elegans. They are divided into four structural domains: flexible N- and C-terminal domains, an accessory domain, and a central alpha-helical domain. The N-terminal domain plays a role in activation of fast synchronous release in murine neurons and in isolated chromaffin cells. The C-terminal domain binds to phospholipids and is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro. The accessory domain is necessary for regulating spontaneous release and the central domain is required for all functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.