Thermo Fisher Scientific ZO-3 Polyclonal Antibody
ZO-3 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat, Dog에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 3 publications |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Dog, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from an internal region of the human ZO-3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533256 |
Product Specific Information
Catalog No. 36-4000 was used successfully in the western blot analysis of ZO-3 in MDCK cell lysate.
Target Information
ZO-3 is a member of the membrane-associated guanylate kinase (MAGUK) family and is considered a tight junction-associated protein. Tight junctions form intercellular boundaries between epithelial and endothelial cells. ZO-3 has both structural and signaling roles and contains PDZ, SH3, and GuK domains. ZO-1 forms complexes with either ZO-2 or ZO-3, and these proteins can associate with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton to the junction. ZO-1 expression is often reduced in breast cancer lines, while ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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