Thermo Fisher Scientific GAPVD1 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
SSLDLEGESV SELGAGPSGS NGVEALQLLE HEQATTQDNL DDKLRKFEIR DMMGLTDDRD ISETVSETWS TDVLGSDFDP NIDEDRLQEI AGAAAENMLG SLLCLPGSGS VLL

Highest antigen sequence identity to the following orthologs:

• Mouse: 98%
• Rat: 98%

Target Information

GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), participating in processes such as endocytosis, insulin receptor internalization, and LC2A4/GLUT4 trafficking. It acts as a GEF for RAB31 by exchanging GDP for GTP, regulating LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state, retaining LC2A4/GLUT4 inside cells. Upon insulin stimulation, GAPVD1 translocates to the plasma membrane, releasing LC2A4/GLUT4 from intracellular vesicles. It also contributes to EGFR trafficking and degradation, possibly by promoting ubiquitination and proteasomal degradation. GAPVD1 exhibits GEF activity for Rab5 and GAP activity for Ras.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.