Thermo Fisher Scientific ADAR Monoclonal Antibody (GT1066)

Thermo Fisher Scientific ADAR Monoclonal Antibody (GT1066)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, HeLa, HepG2, A375
• Predicted reactivity: Mouse (80%), Rat (80%)
• Store as a concentrated solution; centrifuge briefly before opening the vial

Target Information

Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), a process known as A-to-I RNA editing. This modification can alter gene expression and protein function through changes in:

• mRNA translation and codon usage
• Pre-mRNA splicing site recognition
• RNA stability and nuclease recognition
• RNA virus genome replication
• microRNA processing and protein-RNA interactions

ADAR edits both viral and cellular RNAs, either at multiple sites (hyper-editing) or specific sites (site-specific editing). Cellular substrates include BLCAP, GRIA2, HTR2C, and GABRA3, with site-specific editing leading to amino acid substitutions and altered protein functions.

Viral substrates include HCV, VSV, MV, HDV, and HIV-1. ADAR exhibits proviral or antiviral effects depending on the virus and editing mechanism. It impairs HCV replication via RNA editing, enhances MV, VSV, and HIV-1 replication through suppression of EIF2AK2/PKR activation, and promotes HIV-1 infectivity by editing adenosines in viral RNA regions. In HDV, ADAR mediates A-to-I editing converting an amber stop codon to tryptophan, enabling synthesis of large delta antigen (L-HDAg) essential for viral assembly. High ADAR1 levels, however, inhibit HDV replication.

(Source: Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음)