Thermo Fisher Scientific FOXO4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Immunocytochemistry (ICC/IF)

10-20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 15 amino acid peptide near the amino terminus of human FOXO4. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FOXO4,uniProtId:P98177-1,ncbiNodeId:9606,antigenRange:1,antigenLength:505,antigenImageFileName:PA5-20975_FOXO4_P98177-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20975_FOXO4_P98177-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154561

Product Specific Information

A suggested positive control is A-20 cell lysate.

PA5-20975 can be used with blocking peptide PEP-1089.

Target Information

FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. It is also known as AFX1 or MLLT7 (Myeloid/Lymphoid or Mixed-Lineage Leukemia (Trithorax Homolog, Translocated To, 7) and is a member of the O class of forkhead/ winged helix family of transcription factors along with FOXO1, FOXO3 and FOXO6. FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. FOXO4 binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling such as IGFBP1. However, the activation of another downstream target of the Insulin/IGF1 pathway, Akt, leads to phosphorylation of FOXO4 on specific Ser/Thr residues. This phosphorylation mediates binding of FOXO4 to 14-3-3 and eventual exit of FOXO4 from the nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.