Thermo Fisher Scientific alpha COP Recombinant Rabbit Monoclonal Antibody (ARC2215)
alpha-COP 단백질을 인식하는 Recombinant Rabbit Monoclonal 항체로, WB, IHC-P, ELISA에 적합. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, Affinity chromatography로 정제. PBS/glycerol buffer에 보존되며 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC2215 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human COPA (P53621) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897862 |
Product Specific Information
- Positive Samples: HeLa, HT-29, MCF7, Mouse lung, Rat brain
- Immunogen sequence:
MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH DGPVRGIDFH KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF
Target Information
In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, representing subunits of the coatomer complex: alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP.
The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. The N-terminal 25 amino acids of alpha-COP encode the bioactive peptide xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone.
Alternative splicing results in multiple splice forms encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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