Thermo Fisher Scientific DNase 1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

Internucleosomal DNA fragmentation following activation of endonucleases is a common endpoint of apoptosis. DNase I, a Ca²⁺/Mg²⁺-dependent endonuclease expressed in mammalian tissues, mediates internucleosomal DNA degradation in apoptotic human cells. DNase I is highly polymorphic, with at least six known alleles. DNase II, an acidic deoxyribonuclease, acts downstream of caspase activation and also contributes to DNA digestion during apoptosis. DNase I cleaves DNA to 5′-phosphodinucleotide and 5′-phosphooligonucleotide products, while DNase II produces 3′-phosphomononucleotide and 3′-phosphooligonucleotide products. DNase II is typically found in the cytoplasm of epithelial cells but is concentrated in the nuclei of lens fibers, whereas DNase I is concentrated in nuclei of epithelial and fiber cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.