Thermo Fisher Scientific VGAT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

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Immunohistochemistry (IHC)

1:100-1:1,000

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Immunocytochemistry (ICC/IF)

1:500

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Product Specifications

Species Reactivity

Chicken, Mouse, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the N-terminal region of rat SLC32A1 conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VGAT,uniProtId:O35458-1,ncbiNodeId:10114,antigenRange:1,antigenLength:525,antigenImageFileName:PA1-4701_VGAT_O35458-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4701_VGAT_O35458-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2301981

Product Specific Information

This antibody is predicted to react with bovine, canine and non-human primate based on 100% sequence homology.

In Western blot, this antibody is specific for the ~53 kDa VGAT protein.

Target Information

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.