Thermo Fisher Scientific Human FGF-acidic (FGF-1) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human FGF-acidic (FGF-1) recombinant protein contains 141 amino acids

Molecular weight

15.8 kDa

Class

Recombinant

Type

Protein

Purity

≥95% by SDS-PAGE and HPLC

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

5mM sodium phosphate, pH 8, with 100mM NaCl

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 5 mM Sodium Phosphate, pH 8.0, to a concentration of less than 0.5 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.

Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 10 months at -80°C to -20°C. Avoid repeated freeze/thaw cycles.

Target Information

FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.