Thermo Fisher Scientific CD112 (Nectin-2) Monoclonal Antibody (R2.477), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R2.477

Conjugate

APC APC APC

View additional formats

• PE
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573157

Product Specific Information

Description: This R2.477 monoclonal antibody reacts with human CD112 (also known as Nectin-2 or Poliovirus Receptor Related 2), a 60-65 kDa immunoglobulin-like transmembrane cell adhesion molecule. This cell surface molecule is expressed on hematopoietic cells, endothelial, epithelial, as well as tumor cell lines such as Jurkat, U937, and MCF7. CD112 is a ligand for DNAM-1 (CD226), a leukocyte adhesion molecule expressed on natural killer (NK) cells. Interaction between CD112 and DNAM-1 promotes NK cytotoxic activity, leading to tumor cell lysis. In addition to its role in tumor suppression, CD112 plays a cooperative role in cell-cell adhesion, particularly in the formation of adherens junctions between neighboring epithelial cells and fibroblasts. CD112 can form homophilic and heterophilic interactions to regulate contact inhibition of cell movement and proliferation. Finally, CD112 has been reported to be a receptor for herpesvirus entry into cells.

This monoclonal antibody has been reported to block cell adhesion and herpesvirus entry.

Applications Reported: This R2.477 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R2.477 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.