Thermo Fisher Scientific ADFP Polyclonal Antibody
Rabbit polyclonal antibody targeting human ADFP (Adipophilin). Validated for WB, IHC-P, ICC/IF, and Flow Cytometry. Recognizes amino acids 186–214 of ADFP. Supplied as liquid, 0.25 mg/mL in PBS with 0.09% sodium azide. Suitable for detecting lipid drop...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:100 | – |
| Immunocytochemistry (ICC/IF) | – | View 2 publications |
| Flow Cytometry (Flow) | 1:10–1:50 | – |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 186–214 of human ADFP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2542542 |
Target Information
Milk lipid globules from humans, cows, and rats contain a protein known as adipocyte differentiation-related protein (ADFP). This protein is associated with the globule surface membrane material and is a major component of the detergent-insoluble complex containing butyrophilin and xanthine oxidase.
ADFP (Adipophilin) is expressed in various cultured cell lines, including fibroblasts, endothelial, and epithelial cells. In tissues, expression is restricted to specialized cells such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells, and steatotic hepatocytes in alcoholic liver cirrhosis.
ADFP serves as a potential marker for identifying differentiated cells containing lipid droplets and for studying diseases related to fat-accumulating cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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