Thermo Fisher Scientific IDH1 Monoclonal Antibody (OTI2H9)
IDH1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB 및 ICC/IF에 최적화됨. 인간 및 생쥐 반응성, 고순도 친화 크로마토그래피 정제, 안정한 액상 형태로 연구용 적합. -20°C 보관.
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Thermo Fisher Scientific IDH1 Monoclonal Antibody (OTI2H9)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H9 |
| Immunogen | Full-length human IDH1 protein expressed in 293T cells transfected with human IDH1 expression vector |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724497 |
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).
Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases localized to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases — one mitochondrial and one predominantly cytosolic.
Each NADP(+)-dependent isozyme is a homodimer. The encoded protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes, containing the PTS-1 peroxisomal targeting signal sequence.
Its presence in peroxisomes suggests roles in NADPH regeneration for intraperoxisomal reductions and other peroxisomal reactions consuming 2-oxoglutarate. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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