Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 496 / 615 / 737 / 805
• Brilliant Violet 480 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 555 / 585 / 610-30S / 660-40S / 690 / 760
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 570 / 590 / 660
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600
• Custom conjugation available

Product Specific Information

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, and sulfated sialylated glycoproteins such as CD34, glycam-1, and MAdCAM-1, mediating lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of lymphoid tissues.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test
• Recommended Cell Count: 10⁵–10⁸ cells/test

Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

• Excitation: 407 nm
• Emission: 423 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor. It includes:

• C-type lectin binding domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Transmembrane and cytoplasmic regions

CD62L is constitutively expressed on leukocytes including lymphocytes (except some memory T cells), monocytes, and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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