Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Polyclonal Antibody
인간 ZAP-70/Syk의 인산화 형태(Tyr319, Tyr352)를 인식하는 Rabbit Polyclonal 항체로, WB 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. T세포 수용체 활성화 연구에 적합한 연구용 항체.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pTyr319 of human Zap-70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 56 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10983290 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B between SH2 and kinase domains. Phosphorylation of Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while Tyr292 phosphorylation attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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