
Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgA Secondary Antibody, HRP
HRP 결합 F(ab')2 Goat anti-Human IgA 2차 항체로, ELISA, Western blot, IHC에 사용 가능. 인간 IgA에 특이적으로 반응하며 비특이적 결합이 최소화됨. 동결건조 형태로 제공되며 재수화 후 안정적인 신호 증폭 성능 제공.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgA Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgA |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535927 |
| Target | IgA |
| Antibody Form | F(ab`)2 Fragment |
Additional Formats
Product Specific Information
- Each lot’s sensitivity is confirmed using ELISA, and specificity is verified by immunoelectrophoresis (IEP).
- Rehydrate with 0.55 mL of deionized water and allow to stand for at least 30 minutes at room temperature.
- Centrifuge to remove particulates if necessary. Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins.
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
Typically, these antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified via immunoaffinity chromatography, fragmentation, or label conjugation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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