Thermo Fisher Scientific Aminoacylase Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1:5,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 1–4 µL/mg of lysate

Product Specifications

Product Specific Information

• This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
• This antibody has specificity for Human Aminoacylase 1 (ACY1).

Target Information

ACY1 encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group. It functions in the catabolism and salvage of acylated amino acids.
This gene is located on chromosome 3p21.1, a region often reduced to homozygosity in small-cell lung cancer (SCLC), where its expression may be reduced or undetectable.
The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart and represents the first member of a new family of zinc-binding enzymes.
Mutations in ACY1 cause aminoacylase-1 deficiency, a metabolic disorder characterized by CNS defects and increased urinary excretion of N-acetylated amino acids.
Alternative splicing results in multiple transcript variants, and read-through transcription occurs between ACY1 and the upstream ABHD14A gene. A related pseudogene is found on chromosome 18.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.