Thermo Fisher Scientific Phospho-PRAS40 (Thr356) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Fruit fly

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen Affinity Chromatography

Storage buffer

10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807490

Product Specific Information

Antigen Affinity Purified from Pooled Serum

Target Information

The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.