Thermo Fisher Scientific Phospho-FoxO1 (Ser329) Polyclonal Antibody
Phospho-FoxO1 (Ser329) 폴리클로날 항체로 Western blot, IHC, ICC, ELISA 등에 사용 가능. Rabbit IgG 유래로 Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관. FOXO1 인산화 상태 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Ser329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
FOXO1 (FKHR, Forkhead Box O1) is a member of the forkhead homeotic gene family of transcription factors.
FOXO1 can act as a coactivator or corepressor of nuclear receptor activity through the LXXLL motif in its C-terminal region.
It is implicated in alveolar rhabdomyosarcoma via association with PAX3 and plays roles in autophagy, myogenic differentiation, and tumor suppression.
FOXO1 is a primary target of insulin signaling, regulating metabolic homeostasis under oxidative stress.
It binds to insulin response elements (IRE) and Daf-16 family binding elements (DBE), influencing redox balance, osteoblast regulation, bone mass control, and glucose metabolism.
In hepatocytes, FOXO1 promotes gluconeogenesis by activating genes such as IGFBP1, G6PC, and PPCK1 in cooperation with PPARGC1A.
It acts downstream of CDK1, PKB/AKT1, and SKT4/MST1 in regulating cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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