Thermo Fisher Scientific c-Raf Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

an 19 amino acid peptide near the amino terminus of human C-raf. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Raf,uniProtId:P04049-1,ncbiNodeId:9606,antigenRange:1,antigenLength:648,antigenImageFileName:PA5-20970_c-Raf_P04049-1_Chicken.svg,antigenImageFileNamePDP:PA5-20970_c-Raf_P04049-1_Chicken_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153754

Product Specific Information

A suggested positive control is NIH-3T3 cell lysate.

PA5-20970 can be used with blocking peptide PEP-1084.

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.