Thermo Fisher Scientific CD147 Recombinant Rabbit Monoclonal Antibody (JF1-045)
Thermo Fisher의 CD147 Recombinant Rabbit Monoclonal Antibody (JF1-045)은 인간, 마우스, 랫트 시료에 반응하는 고감도 항체입니다. Western blot, IHC, ICC/IF에 최적화되어 있으며, 재조합 기술로 생산되어 로트 간 일관성과 높은 특이성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF1-045 |
| Immunogen | Synthetic peptide within Human CD147 aa 171–204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809811 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
CD147 (also known as basigin, neurothelin, OX-47, 5A11, CE9, M6, EMMPRIN, extracellular matrix metalloproteinase inducer, TCSF) is a 50–60 kDa glycoprotein with multiple glycosylated forms.
It is a plasma membrane protein important in spermatogenesis, embryo implantation, neural network formation, and tumor progression.
CD147 is a member of the immunoglobulin superfamily and is highly expressed in metabolically active cells such as lymphoblasts, inflammatory cells, brown adipocytes, and malignant tumor cells.
Functions include:
- Facilitating cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases
- Regulating integrin functions
- Roles in cell development, activation, fetal development, and retinal function
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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