Thermo Fisher Scientific KIAA1524 Monoclonal Antibody (OTI4F5), TrueMAB
인간 KIAA1524(CIP2A)을 인식하는 단클론 항체로 Western blot에 최적화됨. Mouse IgG1 형식이며, 동결건조 형태로 제공. 재구성 후 약 1 mg/mL 농도로 사용 가능. PP2A 억제 및 c-Myc 안정화 연구에 적합.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 635–905 of human KIAA1524 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Inhibition of protein phosphatase 2A (PP2A) activity is a prerequisite for human cell transformation. The protein, Cancerous Inhibitor of PP2A (CIP2A), interacts with the oncogenic transcription factor c-Myc, inhibiting PP2A activity toward c-Myc serine 62 (S62) and preventing c-Myc proteolytic degradation.
CIP2A promotes anchorage-independent cell growth and tumor formation, and its overexpression is observed in head and neck squamous cell carcinoma (HNSCC) and colon cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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