Thermo Fisher Scientific Cdc25A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 86-328 of CDC25A (NP_0017802) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cdc25A,uniProtId:P30304-1,ncbiNodeId:9606,antigenRange:86-328,antigenLength:524,antigenImageFileName:PA5-109500_Cdc25A_P30304-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109500_Cdc25A_P30304-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.28 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2854911

Product Specific Information

Immunogen sequence: LDSPGPLDSK ENLENPMRRI HSLPQKLLGC SPALKRSHSD SLDHDIFQLI DPDENKENEA FEFKKPVRPV SRGCLHSHGL QEGKDLFTQR QNSAPARMLS SNERDSSEPG NFIPLFTPQS PVTATLSDED DGFVDLLDGE NLKNEEETPS CMASLWTAPL VMRTTNLDNR CKLFDSPSLC SSSTRSVLKR PERSQEESPP GSTKRRKSMS GASPKESTNP EKAHETLHQS LSLASSPKGT IEN

Target Information

CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.