Thermo Fisher Scientific Cul4B Polyclonal Antibody
Thermo Fisher Scientific Cul4B Polyclonal Antibody는 인간 및 생쥐 시료에 반응하는 rabbit IgG 기반 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도로 액상 형태로 제공됩니다. DNA 손상 반응 및 mTOR 경로 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 863 and 913 of human Cullin 4B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
CUL4B is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes that mediate ubiquitination and proteasomal degradation of target proteins. It acts as a scaffold protein, positioning substrates and ubiquitin-conjugating enzymes for catalysis.
CUL4B participates in polyubiquitination of CDT1 and histones H2A, H3, and H4 during radiation-induced DNA damage response. It is targeted to UV-damaged chromatin by DDB2 and plays a role in DNA repair and replication.
Additionally, CUL4B is required for cyclin E ubiquitination and normal G1 cell cycle progression, and it regulates the mTOR pathway controlling cell growth and metabolism. This regulation depends on 26S proteasome function and interaction between CUL4B and MLST8.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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