Thermo Fisher Scientific Phospho-Cyclin B1 (Ser128) Polyclonal Antibody
Cyclin B1의 Ser128 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal 항체. WB, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫드 등 다양한 종에 반응. 연구용으로 세포주기 조절 및 암 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CCNB1 (Accession P14635), corresponding to amino acid residues around phosphorylated Ser128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903190 |
Product Specific Information
This antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser128.
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts exist: one constitutively expressed and another cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is rapidly degraded at the end of mitosis via APC-dependent mechanisms. Overexpression of Cyclin B1 is observed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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