Thermo Fisher Scientific Phospho-Cyclin B1 (Ser133) Polyclonal Antibody
Cyclin B1의 Ser133 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF에 적합하며 Human, Mouse, Rat 반응성. 고순도 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CCNB1 (Accession P14635), corresponding to amino acid residues around phosphorylated Ser133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903191 |
Product Specific Information
This antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser133.
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and essential for control of the cell cycle at the G2/M transition. It forms a complex with p34 (cdc2) to create the maturation-promoting factor (MPF). Two alternative transcripts exist: one constitutively expressed and one cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is destroyed at the end of mitosis via APC-dependent mechanisms. Overexpression of Cyclin B1 has been observed in breast, prostate, and non-small cell lung cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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