Thermo Fisher Scientific Zap-70 Polyclonal Antibody
Human ZAP-70 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. T세포 수용체 활성화 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
- Publications: [참고 없음]
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200–1:500
- Publications: [참고 없음]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ZAP70. Recombinant protein control fragment (Product #RP-95380) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789224 |
Product Specific Information
Immunogen sequence:
LIYCLKEACP NSSASNASGA AAPTLPAHPS TLTHPQRRID TLNSDGYTPE PARITSPDKP RPMPMDTSVY ESPYSDPEEL KDKKLFLKRD NLLIADIELG CGNFGSVRQG VYRMRKKQID VAIKVLKQGT EKADTEEMMR
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor (TCR) activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region.
Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while phosphorylation at tyrosine 292 terminates transient activation and attenuates signaling.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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