Thermo Fisher Scientific Phospho-c-Raf (Thr269) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phospho-c-Raf (Thr269) in human samples. Suitable for dot blot applications. Purified by Protein A and antigen affinity chromatography. Supplied as liquid form in PBS buffer with sodium azide. For research use only.
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Applications
- Dot blot (DB)
Tested Dilution
- 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T269 of human RAF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984075 |
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression related to the cell division cycle, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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