Thermo Fisher Scientific Claudin 4 Polyclonal Antibody (ZMD.306)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 36 publications 36 publications

Immunohistochemistry (IHC)

-

View 32 publications 32 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 20 publications 20 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

1-3 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Dog, Hamster, Human, Mouse, Non-human primate, Pig, Plant, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

ZMD.306

Immunogen

Synthetic peptide derived from the C-terminal region of mouse Claudin-4 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Claudin 4,uniProtId:O35054-1,ncbiNodeId:10090,antigenRange:210,antigenLength:210,antigenImageFileName:36-4800_Claudin_4_O35054-1_Rabbit.svg,antigenImageFileNamePDP:36-4800_Claudin_4_O35054-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533262

Product Specific Information

Reactivity has been confirmed with mouse and rat kidney homogenates. On western blots a 22 kDa band is observed and a 44 kDa band representing dimerized protein may be observed.

Target Information

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.