Thermo Fisher Scientific DKK3 Polyclonal Antibody
인간 DKK3 단백질을 인식하는 goat polyclonal antibody로, WB 및 IHC(P) 실험에 적합합니다. 합성 펩타이드 항원을 사용하여 제작되었으며, 고순도 및 안정적 액상 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (AAAALLGGEEI) corresponding to the C-terminus amino acids of DKK3 (aa 340–350) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10980497 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and two conserved cysteine-rich domains, separated by a linker region containing 10 cysteine residues each.
The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane.
The linker region contains 50–55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids.
All DKKs have potential sites for cleavage by furin-type proteases.
Northern blot analysis shows wide expression of the DKK3 transcript, with highest levels in heart, brain, and spinal cord.
In situ hybridization reveals highest expression in mouse brain, eye, and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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