Thermo Fisher Scientific TCP1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human TCP1, suitable for WB, IHC, and IP applications. Recognizes residues 450–500 of TCP1. Liquid form, unconjugated, 0.20 mg/mL concentration. Predicted to react with rat and bovine. For research use only.
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Thermo Fisher Scientific TCP1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 450 and 500 of Human T-Complex 1 (TCP1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine
Target Information
The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.
Chaperonin proteins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly, sharing characteristics with Heat Shock Protein 70 (HSP70) such as high abundance, induction by environmental stress, and ATPase activity.
The chaperonin family includes mitochondrial HSP60, E. coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55 but only minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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