Thermo Fisher Scientific TPH1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
• Predicted reactivity: Mouse (96%), Rat (96%), Japanese Medaka (89%), Xenopus laevis (90%), Dog (99%), Rabbit (98%), Chicken (93%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons originating in the Raphe nuclei of the brain, while melatonin synthesis takes place in the pineal gland. Although TPH catalyzes the same reaction in both tissues, TPH activity is rate-limiting for serotonin but not for melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca²⁺/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 within the regulatory domain of TPH.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.