Thermo Fisher Scientific NR3C1 Monoclonal Antibody (OTI1B11), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-Dependent

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids acting as both a transcription factor and a regulator of other transcription factors. It forms cytoplasmic complexes with heat shock proteins and immunophilins and translocates to the nucleus upon ligand binding.
Expression has been detected in various heart tissues including atria, ventricles, aorta, and septum.
Alternate splicing and promoter usage result in multiple transcript variants and isoforms.
Mutations in NR3C1 can cause glucocorticoid resistance. The receptor binds steroids such as dexamethasone with nanomolar affinity and requires chaperone complexes for activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.