Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Mouse Monoclonal Antibody (CR55T33)

Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Mouse Monoclonal Antibody (CR55T33)

Applications and Tested Dilution

Product Specifications

Additional Formats

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 647
• Custom conjugation available upon request

Product Specific Information

Recombinant antibodies are produced using genes encoding the desired antibody, cloned into an expression vector and expressed in vitro. These antibodies offer improved specificity and lot-to-lot consistency.

Applications Tested:
The CR55T33 clone has been tested for flow cytometry at 0.5 µg per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be empirically determined (typically 10⁵–10⁸ cells/test). It is recommended to titrate the antibody for optimal performance.

Target Information

Histone H2A.X (H2AX) is a core histone protein involved in nucleosome formation. DNA double-strand breaks trigger phosphorylation of serine 139, forming γ-H2AX, which can be detected by Western blot or immunofluorescence to assess DNA damage frequency.

Phosphorylation is mediated by phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR. H2A.X consists of 143 amino acids and is synthesized during G1 and S-phase. Its mRNA exists in two forms: one polyadenylated (~1600 bases) and one non-polyadenylated (~575 bases). The gene maps to chromosome 11q23.2-q23.3 and plays a key role in DNA structure and repair by regulating MDC1 binding at damage sites.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.