Invitrogen™

MC1061/P3 Chemically Competent E. coli

MC1061/P3 Chemically Competent E. coli are used for highly efficient transformations that require the P3 episome for selection and maintenance자세히 알아보기

MC1061/P3 Chemically Competent E. coli are used for highly efficient transformations that require the P3 episome for selection and maintenance of vectors encoding the tyrosine tRNA suppressor (synthetic supF gene, such as pCDM8, pcDNA1.1, or any other _sup_F-containing vector).

MC1061 E. coli cells were used by Hanahan and coworkers to engineer the DH10B strain. Among the replaced genes by adding mutations were rec_A1 (inhibiting the homologous recombination system), _end_A1, and _lac_ZΔM15. Thus, this strain with its intact homologous recombination system is used in applications where recombination is needed, such as in genetically engineering _E. coli by recombination or plasmid-phage recombination.

MC1061/P3 Chemically Competent E. coli offer:
• Transformation efficiencies of >1 x 108 cfu/μg
• P3 plasmid for selection of _sup_F-containing plasmids
• _hsd_R for efficient transformation of unmethylated DNA from PCR amplifications

*P3 plasmid
*The P3 plasmid is a low-copy-number, 60 kb plasmid that carries kanamycin, tetracycline, and ampicillin resistance markers. The kanamycin gene is fully active and used to select for cells carrying P3. The tetracycline and ampicillin genes carry amber mutations, which render the genes inactive during normal growth and replication of the bacteria. Upon transformation of a vector carrying the suppressor F gene, the amber mutations in the tetracycline and ampicillin genes on the P3 plasmid are suppressed, and the E. coli are resistant to these antibiotics.

Note: The spontaneous reversion rate for the amber mutation in the ampicillin marker is 5%, while the reversion rate for the amber mutation in the tetracycline marker is 1%.To minimizing the reversion rates, use tetracycline and ampicillin together for selection of E. coli (P3) cells transformed with the SupF plasmid.

Genotype
F–hsd_R(rK–, mK+) _ara_D139 Δ(_ara_ABC_-leu)7679 _gal_U _gal_K Δ_lac_X74 _rps_L(StrR) _thi mcr_B / P3: KanR AmpR (am) TetR (am)

Find the strain and format that fits your needs
We offer DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
The TOP10/P3 strain could be used where recombination is not needed.
Strains are available in several MultiShot formats for high throughput applications.
Explore bacterial growth media formats.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선No

메틸화 DNA 클로닝Yes (hsd)

형질전환 효율 수준Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Streptomycin, Kanamycin, Ampicillin, Tetracycline)

불안정 DNA 클로닝No

블루/화이트 스크리닝No

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소No

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

수량5 x 300 μL

Unit SizeEach