Invitrogen™

MegaX DH10B T1R Electrocomp™ Cells

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3자세히 알아보기

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA, making MegaX DH10B T1R cells ideal for highly demanding cloning or cDNA and gDNA library construction applications.

The DH10B T1R strain has all the benefits of DH10B genotype, with the addition of the ton_A (also known as _fhu_A) genotype that confers resistance to T1 and T5 phage infection. The inclusion of the _ton_A genotype offers added security to protect valuable clones and libraries. MegaX DH10B T1R cells carry mutations in the methylation-dependent _E. coli K12 restriction system (mcr_A, _mcr_BC, and _mrr) allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. This strain also has the _lac_ZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. Finally, the _end_A1 mutation makes DH10B T1R an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

MegaX DH10B T1R Electrocomp Cells offer:
• Highest transformation efficiency of >3 x 1010 cfu/μg
• ton_A (_fhuA) genotype to confer resistance to T1 and T5 phage
• lac_ZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• _mcr_A genotypic marker and the _mcr_BC, _mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
• _end_A1 mutation enables increased plasmid yield and quantity
• _rec_A1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: A high-voltage electroporation apparatus is required.

Genotype
F–mcr_A Δ(_mrr_-_hsd_RMS-_mcr_BC) φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _end_A1 _ara_D139 Δ(_ara_-_leu)7697 _gal_U _gal_K λ_rps_L(StrR) _nup_G _ton_A

Find the strain and format that fits your needs
We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
Electroporation cuvettes are available in 0.1 cm, 0.2 cm, and 0.4 cm gaps.
Strains are available in several MultiShot formats for high throughput applications.
Explore bacterial growth media formats.

사양

제품 유형Electrocompetent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준High Efficiency (>3 x 1010 cfu/μg)

항생제 내성 박테리아Yes (Streptomycin)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

플라스미드May be used for plasmids >100 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)Yes

종E. coli (K12)

형식Tube

제품라인Electrocomp™

수량5 x 100 μL

Unit SizeEach