Thermo Fisher Scientific Lamin-A Polyclonal Antibody
Lamin A 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관합니다. 인간 및 생쥐 반응성이 확인되었고, 형광 또는 면역염색 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 400 and 450 of human Lamin A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For ICC: formaldehyde fixation and permeabilization with Triton X-100 recommended
- For IHC: epitope retrieval with citrate buffer (pH 6.0) recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Rat and Pig
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic side of the nuclear membrane.
Two main subtypes of nuclear lamins can be distinguished: A-type and B-type lamins.
A-type lamins (lamin A, lamin C, lamin Adel 10) arise from the same gene by alternative splicing, while B-type lamins (lamin B1, lamin B2) are encoded by distinct genes.
Mutations in A-type lamins are associated with several rare but dominant genetic disorders, including:
- Emery-Dreifuss muscular dystrophy
- Dilated cardiomyopathy with conduction-system disease
- Dunnigan-type familial partial lipodystrophy
A-type lamins are expressed during cell differentiation and interact with chromatin, suggesting a regulatory role in differential gene expression.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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