Thermo Fisher Scientific MBD1 Recombinant Rabbit Monoclonal Antibody (10B10)
Recombinant rabbit monoclonal antibody for MBD1 detection. Suitable for ICC/IF and ELISA assays. High specificity for human MBD1 with affinity-purified formulation. Provided in PBS buffer with glycerol for stable storage at -20°C. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10B10 |
| Immunogen | Synthesized peptide derived from human MBD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3092677 |
Target Information
Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It functions as a transcriptional repressor that binds CpG islands in promoters where DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG produced by DNA damage via methylmethanesulfonate (MMS). MBD1 contributes to gene silencing by recruiting AFT7IP, which in turn recruits histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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